custom- rabbit polyclonal anti- p37  (ABclonal Biotechnology)

Journal: Cell reports

Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms

doi: 10.1016/j.celrep.2024.115113

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Ubiquitin (P37) , Cell Signaling Technology , 58395; RRID:AB_3075532.

Techniques: Synthesized, Virus, Plasmid Preparation, shRNA, Recombinant, Transfection, Injection, Software, Imaging

Journal: Cancers

Article Title: Chemokine CXCL12 Activates CXC Receptor 4 Metastasis Signaling Through the Upregulation of a CXCL12/CXCR4/MDMX (MDM4) Axis

doi: 10.3390/cancers16244194

Figure Lengend Snippet: CXCL12 addition does not increase MDMX protein half-life following cycloheximide or MG132 treatment. ( A ) Immunoblot analysis of lysates from MDA-MB-231 cells treated with CXCL12 (50 ng/mL) for 30 min followed by cycloheximide (CHX) or DMSO for 40 or 80 min. Cells were harvested and lysed in CHAPS lysis buffer and subjected to immunoblotting to probe for MDM2 and MDMX. Actin was probed as a loading control. ( B , C ) Evaluation of Western blot band density was carried out using ImageJ and Prism software. Error bars represent SD. * p < 0.05, NS = non-significant. ( D ) HCT116 p53-/- cells were transfected with pcDNA3-MDMX for 24 h and then treated with CXCL12 (50 ng/mL) for 30 min followed by MG132 or DMSO for 40 or 80 min. Cells were harvested and lysed in CHAPS lysis buffer and subjected to immunoblotting to probe for MDM2, MDMX, and Ubiquitin. Actin was probed as a loading control. ( E , F ) Evaluation of Western blot band density was carried out using ImageJ and Prism 10 software. Error bars represent SD. * p < 0.05, NS = non-significant.

Article Snippet: MDM2 R&D Systems Cat no. AF1244 (1:2000) Rabbit in 1% milk PBST, MDMX, 53BP1 Cell Signaling (1:1000) Rabbit in 1% milk TBST, PARP-1 Proteintech (1:1000) Rabbit in TBST, GST Santa Cruz Technologies (1:2000) Mouse in 1% milk PBST, Ubiquitin Cell Signaling Rabbit Polyclonal Ab (1:10,000) in 5% BSA PBST, CXCR4 mouse antibody Proteintech (1:2000) Cat No. 60042-1-Ig in 1% milk PBST, phospho-AKT S473 Cell Signaling (1:1000) Cat No. 9217S Rabbit 5% BSA in TBST, and MDMX (Cat No. 17914-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Western Blot, Lysis, Control, Software, Transfection, Ubiquitin Proteomics

Journal: Cancer Science

Article Title: Downregulated mitochondrial transcription factor A enhances mycoplasma infection to promote the metastasis of hepatocellular carcinoma

doi: 10.1111/cas.15715

Figure Lengend Snippet: Primary Abs used for western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF)

Article Snippet: Custom‐made rabbit polyclonal anti‐P37 , ABclonal , IHC 1:1000, WB 1:1000.

Techniques: Western Blot, Immunohistochemistry, Immunofluorescence, Concentration Assay